Scientific corner

Adsorption of Soluble Immunoglobulin-Type Adhesion Molecules to Cellulose Acetate Beads

Shoichi Nishise 1Yuji Takeda 2Hidetoshi Nara 2Yasuhiko Abe 1Yu Sasaki 1Hironobu Asao 2Yoshiyuki Ueno 1 , Ther Apher Dial. 2018 Jun;22(3):261-265.

These results suggest that independent of incubation temperature, sICAM-1 and sVCAM-1 are likely to adsorb CA beads. These molecules may be a new index for predicting the therapeutic effects of GMA.

Scientific corner

Granulocyte and monocyte adsorptive apheresis ameliorates sepsis in rats.

Shuai Ma 1Qingqing Xu 1Bo Deng 1Yin Zheng 2Hongyan Tian 1Li Wang 3Feng Ding 4 , Intensive Care Med Exp. 2017 Dec;5(1):18.

This study showed that selective granulocyte and monocyte adsorption with cellulose acetate beads might ameliorate cecal ligation and puncture (CLP)-induced sepsis and improve survival and organ function.

Scientific corner

Effect of Cellulose Acetate Beads on Interleukin-23 Release

Shoichi Nishise 1Yasuhiko Abe 1Eiki Nomura 2Takeshi Sato 1Yu Sasaki 1Daisuke Iwano 1Kazuya Yoshizawa 1Makoto Yagi 1Kazuhiro Sakuta 1Yoshiyuki Ueno 1 , Ther Apher Dial. 2016 Aug;20(4):354-9.

 In conclusion, CA beads inhibited IL-23 release from adsorbed GMs. The biological effects of this decrease in IL-23 release during GM adsorption to CA beads need further clarification.

Scientific corner

Effect of cellulose acetate beads on the release of Transforming Growth Factor-β

Shoichi Nishise 1Yasuhiko Abe 1Eiki Nomura 1Takeshi Sato 1Yu Sasaki 1Daisuke Iwano 1Makoto Yagi 1Kazuhiro Sakuta 1Rika Shibuya 1Naoko Mizumoto 1Nana Kanno 1Yoshiyuki Ueno 1 , Ther Apher Dial. 2015 Aug;19(4):330-5.

In conclusion, CA beads inhibited the release of TGF-β from adsorbed platelets. The biological effects of this reduction of TGF-β release during platelet adsorption to CA beads need further clarification.

Scientific corner

Human neutrophil Fc receptor-mediated adhesion under flow: a hollow fibre model of intravascular arrest

C D’Arrigo 1J J Candal-CoutoM GreerD J VealeJ M Woof Clin Exp Immunol 1995 Apr;100(1):173-9. doi: 10.1111/j.1365-2249.1995.tb03620.x.

Human polymorphonuclear cells (PMN) were found to adhere to a novel model of blood vessel wall-associated IgG. The internal surfaces of cellulose acetate hollow fibres, of comparable internal diameter to small blood vessels, were coated with normal serum human IgG, heat-aggregated IgG (HAIgG), laminin or fibrinogen. Under conditions of flow mimicking those in a small vessel, PMN were found to adhere markedly only to immunoglobulin-coated fibres. Arrest on HAIgG was inhibited by excess soluble IgG but not by bovine serum albumin (BSA), demonstrating that the adhesion was IgG-specific and presumably mediated by Fc gamma R on the PMN surface. Pre-adsorption of serum components onto HAIgG-coated fibres enhanced PMN arrest, due most probably to fixation of complement components by immobilized HAIgG, resulting in additional potential to entrap PMN via complement receptors such as CR3. Treatment of PMN with the regulatory neuropeptide substance P also enhanced adhesion to HAIgG-coated fibres and caused increased surface expression of Fc gamma RI, Fc gamma RII and Fc gamma RIII. A mouse cell line derived from L cells, hR4C6, stably transfected with human Fc gamma RII, was found to adhere under flow to HAIgG-coated fibres, whilst untransfected parent L cells did not. This adhesion was similarly inhibited by excess soluble IgG, confirming the capability of Fc gamma R to mediate cell arrest. The study strongly suggests that Fc gamma R may play an important role in intravascular PMN arrest and we speculate that in inflammatory diseases PMN may adhere via Fc gamma R to immobilized immunoglobulin on the vascular endothelium, with subsequent degranulation and tissue damage.

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