Scientific corner

Physiological heating augments the anti-inflammatory reactions during granulocyte/monocyte apheresis: A in vitro study

Shoichi Nishise 1 2Yuji Takeda 3Yasuhiko Abe 1Yu Sasaki 1Shinichi Saitoh 3Hidetoshi Nara 3Hironobu Asao 3Yoshiyuki Ueno, Ther Apher Dial 2020 Oct 17.

These results indicated that physiological heating of the apheresis carrier augmented the anti-inflammatory reaction in vitro. Thus, heating during GMA may be a new approach for augmenting clinical efficacy.

Scientific corner

Adsorption of Soluble Immunoglobulin-Type Adhesion Molecules to Cellulose Acetate Beads

Shoichi Nishise 1Yuji Takeda 2Hidetoshi Nara 2Yasuhiko Abe 1Yu Sasaki 1Hironobu Asao 2Yoshiyuki Ueno 1 , Ther Apher Dial. 2018 Jun;22(3):261-265.

These results suggest that independent of incubation temperature, sICAM-1 and sVCAM-1 are likely to adsorb CA beads. These molecules may be a new index for predicting the therapeutic effects of GMA.

Scientific corner

Granulocyte and monocyte adsorptive apheresis ameliorates sepsis in rats.

Shuai Ma 1Qingqing Xu 1Bo Deng 1Yin Zheng 2Hongyan Tian 1Li Wang 3Feng Ding 4 , Intensive Care Med Exp. 2017 Dec;5(1):18.

This study showed that selective granulocyte and monocyte adsorption with cellulose acetate beads might ameliorate cecal ligation and puncture (CLP)-induced sepsis and improve survival and organ function.

Scientific corner

Effect of Temperature on Granulocyte and Monocyte Adsorption to Cellulose Acetate Beads.

Shoichi Nishise 1Yuji Takeda 2Yasuhiko Abe 1Yu Sasaki 1Hidetoshi Nara 2Hironobu Asao 2Yoshiyuki Ueno 1 , Ther Apher Dial. 2017 Jun;21(3):248-254.

These results suggest that warming the column during GMA might increase GM adsorption to CA beads, thereby enhancing the clinical efficacy of GMA.

Scientific corner

Effect of Cellulose Acetate Beads on Interleukin-23 Release

Shoichi Nishise 1Yasuhiko Abe 1Eiki Nomura 2Takeshi Sato 1Yu Sasaki 1Daisuke Iwano 1Kazuya Yoshizawa 1Makoto Yagi 1Kazuhiro Sakuta 1Yoshiyuki Ueno 1 , Ther Apher Dial. 2016 Aug;20(4):354-9.

 In conclusion, CA beads inhibited IL-23 release from adsorbed GMs. The biological effects of this decrease in IL-23 release during GM adsorption to CA beads need further clarification.

Scientific corner

Effect of cellulose acetate beads on the release of Transforming Growth Factor-β

Shoichi Nishise 1Yasuhiko Abe 1Eiki Nomura 1Takeshi Sato 1Yu Sasaki 1Daisuke Iwano 1Makoto Yagi 1Kazuhiro Sakuta 1Rika Shibuya 1Naoko Mizumoto 1Nana Kanno 1Yoshiyuki Ueno 1 , Ther Apher Dial. 2015 Aug;19(4):330-5.

In conclusion, CA beads inhibited the release of TGF-β from adsorbed platelets. The biological effects of this reduction of TGF-β release during platelet adsorption to CA beads need further clarification.

Scientific corner

Relationship Between Tumor Necrosis Factor-α Release and Granulocyte and Monocyte Adsorption to Cellulose Acetate Beads

Shoichi Nishise 1Yasuhiko AbeEiki NomuraTakeshi SatoYu SasakiDaisuke IwanoKazuya YoshizawaMakoto YagiYuko NishiseYoshiyuki Ueno, Ther Apher Dial. 2014 Jun;18(3):252-7.

umor necrosis factor-α, (TNF)-α, a proinflammatory cytokine, is produced by activated granulocytes and monocytes (GMs) and implicated as a major factor in inflammatory bowel disease (IBD) pathogenesis. Reduction of TNF-α should improve IBD pathology. GM adsorptive apheresis (GMA) is an effective therapy for inflammatory disorders including IBD. GM adsorption to cellulose acetate (CA) beads induces anti-inflammatory cytokine release, although these effects on TNF-α release are not clarified. We hypothesized that GMA may inhibit TNF-α release. The aim of the present study was to clarify the effects of GM adsorption to CA beads on TNF-α release in vitro. Peripheral blood was incubated with and without CA beads and TNF-α was measured. For comparison, TNF-α was measured in another lipopolysaccharide (LPS)-containing peripheral blood sample incubated similarly. The amount of TNF-α in blood samples incubated with CA beads was significantly higher than in those incubated without beads, although it was significantly lower than TNF-α incubated with LPS-containing sample without beads. The amount of TNF-α after incubation with CA beads positively correlated with GM adsorption ratio. GM adsorption to CA beads induced a small amount of TNF-α release. This is the first report on TNF-α release induced via GM adsorption stimuli. The biological effects of TNF-α release during GM adsorption need to be clarified.

Scientific corner

Involvement of Extracellular Signal-Regulated Kinase in Leukocyte Adsorption-Induced Production of Hepatocyte Growth Factor.

Scientific corner

Granulocyte and monocyte adsorption apheresis for Behçet’s disease in a pregnant woman.

Yuko Higashi 1Mitsuyoshi ShimokawaKazuhiro KawaiTakuro Kanekura, J Dermatol. 2013 Dec;40(12):1042-4.

We present a 39-year-old pregnant woman with Behçet’s disease who was treated successfully with granulocyte and monocyte adsorption apheresis (GMA). There were no complications or adverse effects during her pregnancy and delivery. The neonate manifested no abnormalities.

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