{"id":9054,"date":"2017-07-17T11:34:00","date_gmt":"2017-07-17T09:34:00","guid":{"rendered":"https:\/\/www.adacyte.com\/?post_type=scientific-corner&#038;p=9054"},"modified":"2023-12-21T11:38:22","modified_gmt":"2023-12-21T10:38:22","slug":"functionality-of-immunoglobulin-g-and-immunoglobulin-m-antibody-physisorbed-on-cellulosic-films","status":"publish","type":"scientific-corner","link":"https:\/\/www.adacyte.com\/es\/professional\/scientific-corner\/functionality-of-immunoglobulin-g-and-immunoglobulin-m-antibody-physisorbed-on-cellulosic-films\/","title":{"rendered":"Functionality of Immunoglobulin G and Immunoglobulin M Antibody Physisorbed on Cellulosic Films"},"content":{"rendered":"<div class=\"post-content max-w-987 mx-auto px-20 lg:px-0\">\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Huang+Z&amp;cauthor_id=28770196\">Ziwei Huang<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28770196\/#full-view-affiliation-1\">1<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Raghuwanshi+VS&amp;cauthor_id=28770196\">Vikram Singh Raghuwanshi<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28770196\/#full-view-affiliation-1\">1<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Garnier+G&amp;cauthor_id=28770196\">Gil Garnier<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28770196\/#full-view-affiliation-1\">1<\/a><\/sup>Front Bioeng Biotechnol.\u00a02017 Jul 17:5:41.\u00a0doi: 10.3389\/fbioe.2017.00041<\/p>\n<\/div>\n\n<div class=\"post-content max-w-987 mx-auto px-20 lg:px-0\">\n<p>The functionality and aging mechanism of antibodies physisorbed onto cellulosic films was investigated. Blood grouping antibodies immunoglobulin G (IgG) and immunoglobulin M (IgM) were adsorbed onto smooth cellulose acetate (CAF) and regenerated cellulose (RCF) films. Cellulose films and adsorbed IgG layers were characterized at the air and liquid interface by X-ray and neutron reflectivity (NR), respectively. Cellulose film 208 \u00c5 thick (in air) swell to 386 \u00c5 once equilibrated in water. IgG adsorbs from solution onto cellulose as a partial layer 62 \u00c5 thick. IgG and IgM antibodies were adsorbed onto cellulose and cellulose acetate films, air dried, and aged at room temperature for periods up to 20 days. Antibody functionality and surface hydrophobicity were measured everyday with the size of red blood cell (RBC) agglutinates (using RBC specific to IgG\/IgM) and the water droplet contact angle, respectively. The functionality of the aged IgG\/IgM decreases faster if physisorbed on cellulose than on cellulose acetate and correlates to surface hydrophobicity. IgG physisorbed on RCF or CAF age better and remain functional longer than physisorbed IgM. We found a correlation between antibody stability and hydrogen bond formation ability of the system, evaluated from antibody carbonyl concentration and cellulosic surface hydroxyl concentration. Antibody physisorbs on cellulose by weak dipole forces and hydrogen bonds. Strong hydrogen bonding contributes to the physisorption of antibody on cellulose into a non-functional configuration in which the molecule relaxes by rotation of hydophobic groups toward the air interface.<\/p>\n<\/div>\n\n<div class=\"post-content max-w-987 mx-auto px-20 lg:px-0\">\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28770196\/\">Functionality of Immunoglobulin G and Immunoglobulin M Antibody Physisorbed on Cellulosic Films &#8211; PubMed (nih.gov)<\/a><\/p>\n<\/div>\n\n<div class=\"post-content max-w-987 mx-auto px-20 lg:px-0\">\n<p><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5511829\/\">Functionality of Immunoglobulin G and Immunoglobulin M Antibody Physisorbed on Cellulosic Films &#8211; PMC (nih.gov)<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Ziwei Huang\u00a01,\u00a0Vikram Singh Raghuwanshi\u00a01,\u00a0Gil Garnier\u00a01Front Bioeng Biotechnol.\u00a02017 Jul 17:5:41.\u00a0doi: 10.3389\/fbioe.2017.00041 The functionality and aging mechanism of antibodies physisorbed onto cellulosic films was investigated. Blood grouping antibodies immunoglobulin G (IgG) and immunoglobulin M (IgM) were adsorbed onto smooth cellulose acetate (CAF) and regenerated cellulose (RCF) films. Cellulose films and adsorbed IgG layers were characterized at the [&hellip;]<\/p>\n","protected":false},"template":"","scientific_tags":[103,398,56,157,424,761,74],"class_list":["post-9054","scientific-corner","type-scientific-corner","status-publish","hentry","scientific_tags-adsorption","scientific_tags-antibody","scientific_tags-cellulose","scientific_tags-efficacy","scientific_tags-gma","scientific_tags-immunoglobulin","scientific_tags-leukocytapheresis"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Functionality of Immunoglobulin G and Immunoglobulin M Antibody Physisorbed on Cellulosic Films - Adacyte<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/functionality-of-immunoglobulin-g-and-immunoglobulin-m-antibody-physisorbed-on-cellulosic-films\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Functionality of Immunoglobulin G and Immunoglobulin M Antibody Physisorbed on Cellulosic Films - Adacyte\" \/>\n<meta property=\"og:description\" content=\"Ziwei Huang\u00a01,\u00a0Vikram Singh Raghuwanshi\u00a01,\u00a0Gil Garnier\u00a01Front Bioeng Biotechnol.\u00a02017 Jul 17:5:41.\u00a0doi: 10.3389\/fbioe.2017.00041 The functionality and aging mechanism of antibodies physisorbed onto cellulosic films was investigated. Blood grouping antibodies immunoglobulin G (IgG) and immunoglobulin M (IgM) were adsorbed onto smooth cellulose acetate (CAF) and regenerated cellulose (RCF) films. Cellulose films and adsorbed IgG layers were characterized at the [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/functionality-of-immunoglobulin-g-and-immunoglobulin-m-antibody-physisorbed-on-cellulosic-films\/\" \/>\n<meta property=\"og:site_name\" content=\"Adacyte\" \/>\n<meta property=\"article:modified_time\" content=\"2023-12-21T10:38:22+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Tiempo de lectura\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutos\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/functionality-of-immunoglobulin-g-and-immunoglobulin-m-antibody-physisorbed-on-cellulosic-films\/\",\"url\":\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/functionality-of-immunoglobulin-g-and-immunoglobulin-m-antibody-physisorbed-on-cellulosic-films\/\",\"name\":\"Functionality of Immunoglobulin G and Immunoglobulin M Antibody Physisorbed on Cellulosic Films - Adacyte\",\"isPartOf\":{\"@id\":\"https:\/\/www.adacyte.com\/#website\"},\"datePublished\":\"2017-07-17T09:34:00+00:00\",\"dateModified\":\"2023-12-21T10:38:22+00:00\",\"inLanguage\":\"es\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/functionality-of-immunoglobulin-g-and-immunoglobulin-m-antibody-physisorbed-on-cellulosic-films\/\"]}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.adacyte.com\/#website\",\"url\":\"https:\/\/www.adacyte.com\/\",\"name\":\"Adacyte\",\"description\":\"Adacyte Therapeutics\",\"publisher\":{\"@id\":\"https:\/\/www.adacyte.com\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.adacyte.com\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"es\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.adacyte.com\/#organization\",\"name\":\"Adacyte\",\"url\":\"https:\/\/www.adacyte.com\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"es\",\"@id\":\"https:\/\/www.adacyte.com\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.adacyte.com\/wp-content\/uploads\/2021\/01\/logo-adacyte.svg\",\"contentUrl\":\"https:\/\/www.adacyte.com\/wp-content\/uploads\/2021\/01\/logo-adacyte.svg\",\"width\":165,\"height\":46,\"caption\":\"Adacyte\"},\"image\":{\"@id\":\"https:\/\/www.adacyte.com\/#\/schema\/logo\/image\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Functionality of Immunoglobulin G and Immunoglobulin M Antibody Physisorbed on Cellulosic Films - Adacyte","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.adacyte.com\/professional\/scientific-corner\/functionality-of-immunoglobulin-g-and-immunoglobulin-m-antibody-physisorbed-on-cellulosic-films\/","og_locale":"es_ES","og_type":"article","og_title":"Functionality of Immunoglobulin G and Immunoglobulin M Antibody Physisorbed on Cellulosic Films - Adacyte","og_description":"Ziwei Huang\u00a01,\u00a0Vikram Singh Raghuwanshi\u00a01,\u00a0Gil Garnier\u00a01Front Bioeng Biotechnol.\u00a02017 Jul 17:5:41.\u00a0doi: 10.3389\/fbioe.2017.00041 The functionality and aging mechanism of antibodies physisorbed onto cellulosic films was investigated. Blood grouping antibodies immunoglobulin G (IgG) and immunoglobulin M (IgM) were adsorbed onto smooth cellulose acetate (CAF) and regenerated cellulose (RCF) films. Cellulose films and adsorbed IgG layers were characterized at the [&hellip;]","og_url":"https:\/\/www.adacyte.com\/professional\/scientific-corner\/functionality-of-immunoglobulin-g-and-immunoglobulin-m-antibody-physisorbed-on-cellulosic-films\/","og_site_name":"Adacyte","article_modified_time":"2023-12-21T10:38:22+00:00","twitter_card":"summary_large_image","twitter_misc":{"Tiempo de lectura":"2 minutos"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.adacyte.com\/professional\/scientific-corner\/functionality-of-immunoglobulin-g-and-immunoglobulin-m-antibody-physisorbed-on-cellulosic-films\/","url":"https:\/\/www.adacyte.com\/professional\/scientific-corner\/functionality-of-immunoglobulin-g-and-immunoglobulin-m-antibody-physisorbed-on-cellulosic-films\/","name":"Functionality of Immunoglobulin G and Immunoglobulin M Antibody Physisorbed on Cellulosic Films - Adacyte","isPartOf":{"@id":"https:\/\/www.adacyte.com\/#website"},"datePublished":"2017-07-17T09:34:00+00:00","dateModified":"2023-12-21T10:38:22+00:00","inLanguage":"es","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.adacyte.com\/professional\/scientific-corner\/functionality-of-immunoglobulin-g-and-immunoglobulin-m-antibody-physisorbed-on-cellulosic-films\/"]}]},{"@type":"WebSite","@id":"https:\/\/www.adacyte.com\/#website","url":"https:\/\/www.adacyte.com\/","name":"Adacyte","description":"Adacyte Therapeutics","publisher":{"@id":"https:\/\/www.adacyte.com\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.adacyte.com\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"es"},{"@type":"Organization","@id":"https:\/\/www.adacyte.com\/#organization","name":"Adacyte","url":"https:\/\/www.adacyte.com\/","logo":{"@type":"ImageObject","inLanguage":"es","@id":"https:\/\/www.adacyte.com\/#\/schema\/logo\/image\/","url":"https:\/\/www.adacyte.com\/wp-content\/uploads\/2021\/01\/logo-adacyte.svg","contentUrl":"https:\/\/www.adacyte.com\/wp-content\/uploads\/2021\/01\/logo-adacyte.svg","width":165,"height":46,"caption":"Adacyte"},"image":{"@id":"https:\/\/www.adacyte.com\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/www.adacyte.com\/es\/wp-json\/wp\/v2\/scientific-corner\/9054","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.adacyte.com\/es\/wp-json\/wp\/v2\/scientific-corner"}],"about":[{"href":"https:\/\/www.adacyte.com\/es\/wp-json\/wp\/v2\/types\/scientific-corner"}],"wp:attachment":[{"href":"https:\/\/www.adacyte.com\/es\/wp-json\/wp\/v2\/media?parent=9054"}],"wp:term":[{"taxonomy":"scientific_tags","embeddable":true,"href":"https:\/\/www.adacyte.com\/es\/wp-json\/wp\/v2\/scientific_tags?post=9054"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}