{"id":7016,"date":"2009-04-10T09:51:00","date_gmt":"2009-04-10T09:51:00","guid":{"rendered":"https:\/\/www.adacyte.com\/?post_type=scientific-corner&#038;p=7016"},"modified":"2022-03-24T10:33:47","modified_gmt":"2022-03-24T10:33:47","slug":"granulocyte-and-monocyte-adsorption-apheresis-therapy-modulates-monocyte-derived-dendritic-cell-function-in-patients-with-ulcerative-colitis","status":"publish","type":"scientific-corner","link":"https:\/\/www.adacyte.com\/de\/professional\/scientific-corner\/granulocyte-and-monocyte-adsorption-apheresis-therapy-modulates-monocyte-derived-dendritic-cell-function-in-patients-with-ulcerative-colitis\/","title":{"rendered":"Granulocyte and Monocyte Adsorption Apheresis Therapy Modulates Monocyte-Derived Dendritic Cell Function in Patients With Ulcerative Colitis"},"content":{"rendered":"<div class=\"post-content max-w-987 mx-auto px-20 lg:px-0\">\n<p><a href=\"https:\/\/onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Iwakami%2C+Yuko\">Yuko Iwakami<\/a>,<a href=\"https:\/\/onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Sakuraba%2C+Atsushi\">Atsushi Sakuraba<\/a>,<a href=\"https:\/\/onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Sato%2C+Toshiro\">Toshiro Sato<\/a>,<a href=\"https:\/\/onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Takada%2C+Yasuhiro\">Yasuhiro Takada<\/a>,<a href=\"https:\/\/onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Izumiya%2C+Motoko\">Motoko Izumiya<\/a>,<a href=\"https:\/\/onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Ichikawa%2C+Hitoshi\">Hitoshi Ichikawa<\/a>,<a href=\"https:\/\/onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Hibi%2C+Toshifumi\">Toshifumi Hibi<\/a> Ther Apher Dial <a href=\"https:\/\/onlinelibrary.wiley.com\/toc\/17449987\/2009\/13\/2\">13,&nbsp;2<\/a> (2009);&nbsp;138-146<\/p>\n<\/div>\n\n<div class=\"post-content max-w-987 mx-auto px-20 lg:px-0\">\n<p>The aim of this study was to elucidate the molecular mechanisms responsible for the therapeutic effects of granulocyte and monocyte adsorption apheresis (GMA). We investigated the alterations in circulating monocyte subsets and monocyte-derived dendritic cell (moDC) function after GMA therapy in ulcerative colitis (UC) patients. Eighteen patients with UC were enrolled: 14 patients were responders, and 4 patients were non-responders. Peripheral venous blood was obtained within 5\u2003min before and 5\u2003min after GMA therapy. Flow cytometric analysis for monocyte markers (CD14\/CD16) was then performed. Monocyte-derived dendritic cells were obtained and alterations in their phenotype were analyzed by flow cytometry. Their function was also analyzed in a mixed lymphocyte reaction assay between allo-na\u00efve T lymphocytes. Flow cytometric analysis for intracellular interferon (IFN)-\u03b3 (T-helper 1 cells) and interleukin (IL)-4 (T-helper 2 cells) was then performed for the stimulated T lymphocytes. In patients who responded to GMA, the average numbers of monocytes, especially CD16<sup>+<\/sup>\u00a0monocytes, were significantly decreased after therapy (<em>P<\/em>\u2003&lt;\u20030.05). In responders, post-GMA moDCs expressed significantly lower CD80 and B7-DC, which are one of the stimulation and maturation markers of dendritic cells, compared to pre-GMA moDCs. CD83, CD86 and human leukocytcde antigen-DR also showed a tendency to decrease. In responders, na\u00efve T lymphocytes stimulated with post-GMA moDCs produced significantly less IFN-\u03b3 and IL-4 compared to those stimulated with pre-GMA moDCs. The results of our study show that some of the immunosuppressive effects of GMA therapy may be associated with the modulation of monocyte subsets and moDC function.<\/p>\n<\/div>\n\n<div class=\"post-content max-w-987 mx-auto px-20 lg:px-0\">\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/19379153\/\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/pubmed.ncbi.nlm.nih.gov\/19379153\/<\/a><\/p>\n<\/div>\n\n<div class=\"post-content max-w-987 mx-auto px-20 lg:px-0\">\n<p><a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1744-9987.2009.00668.x\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1744-9987.2009.00668.x<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Yuko Iwakami,Atsushi Sakuraba,Toshiro Sato,Yasuhiro Takada,Motoko Izumiya,Hitoshi Ichikawa,Toshifumi Hibi Ther Apher Dial 13,&nbsp;2 (2009);&nbsp;138-146 The aim of this study was to elucidate the molecular mechanisms responsible for the therapeutic effects of granulocyte and monocyte adsorption apheresis (GMA). We investigated the alterations in circulating monocyte subsets and monocyte-derived dendritic cell (moDC) function after GMA therapy in ulcerative [&hellip;]<\/p>\n","protected":false},"template":"","scientific_tags":[249,284,568,570,569,567,516,424,246,370,173,107,306],"class_list":["post-7016","scientific-corner","type-scientific-corner","status-publish","hentry","scientific_tags-cd14","scientific_tags-cd16","scientific_tags-cd80","scientific_tags-cd83","scientific_tags-cd86","scientific_tags-circulating","scientific_tags-dendritic-cells","scientific_tags-gma","scientific_tags-hla-dr","scientific_tags-immunomodulation","scientific_tags-moa","scientific_tags-monocyte","scientific_tags-ulcerative-colitis"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Granulocyte and Monocyte Adsorption Apheresis Therapy Modulates Monocyte-Derived Dendritic Cell Function in Patients With Ulcerative Colitis - Adacyte<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/granulocyte-and-monocyte-adsorption-apheresis-therapy-modulates-monocyte-derived-dendritic-cell-function-in-patients-with-ulcerative-colitis\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Granulocyte and Monocyte Adsorption Apheresis Therapy Modulates Monocyte-Derived Dendritic Cell Function in Patients With Ulcerative Colitis - Adacyte\" \/>\n<meta property=\"og:description\" content=\"Yuko Iwakami,Atsushi Sakuraba,Toshiro Sato,Yasuhiro Takada,Motoko Izumiya,Hitoshi Ichikawa,Toshifumi Hibi Ther Apher Dial 13,&nbsp;2 (2009);&nbsp;138-146 The aim of this study was to elucidate the molecular mechanisms responsible for the therapeutic effects of granulocyte and monocyte adsorption apheresis (GMA). We investigated the alterations in circulating monocyte subsets and monocyte-derived dendritic cell (moDC) function after GMA therapy in ulcerative [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/granulocyte-and-monocyte-adsorption-apheresis-therapy-modulates-monocyte-derived-dendritic-cell-function-in-patients-with-ulcerative-colitis\/\" \/>\n<meta property=\"og:site_name\" content=\"Adacyte\" \/>\n<meta property=\"article:modified_time\" content=\"2022-03-24T10:33:47+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Gesch\u00e4tzte Lesezeit\" \/>\n\t<meta name=\"twitter:data1\" content=\"2\u00a0Minuten\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/granulocyte-and-monocyte-adsorption-apheresis-therapy-modulates-monocyte-derived-dendritic-cell-function-in-patients-with-ulcerative-colitis\/\",\"url\":\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/granulocyte-and-monocyte-adsorption-apheresis-therapy-modulates-monocyte-derived-dendritic-cell-function-in-patients-with-ulcerative-colitis\/\",\"name\":\"Granulocyte and Monocyte Adsorption Apheresis Therapy Modulates Monocyte-Derived Dendritic Cell Function in Patients With Ulcerative Colitis - Adacyte\",\"isPartOf\":{\"@id\":\"https:\/\/www.adacyte.com\/#website\"},\"datePublished\":\"2009-04-10T09:51:00+00:00\",\"dateModified\":\"2022-03-24T10:33:47+00:00\",\"inLanguage\":\"de\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/granulocyte-and-monocyte-adsorption-apheresis-therapy-modulates-monocyte-derived-dendritic-cell-function-in-patients-with-ulcerative-colitis\/\"]}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.adacyte.com\/#website\",\"url\":\"https:\/\/www.adacyte.com\/\",\"name\":\"Adacyte\",\"description\":\"Adacyte Therapeutics\",\"publisher\":{\"@id\":\"https:\/\/www.adacyte.com\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.adacyte.com\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"de\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.adacyte.com\/#organization\",\"name\":\"Adacyte\",\"url\":\"https:\/\/www.adacyte.com\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"de\",\"@id\":\"https:\/\/www.adacyte.com\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.adacyte.com\/wp-content\/uploads\/2021\/01\/logo-adacyte.svg\",\"contentUrl\":\"https:\/\/www.adacyte.com\/wp-content\/uploads\/2021\/01\/logo-adacyte.svg\",\"width\":165,\"height\":46,\"caption\":\"Adacyte\"},\"image\":{\"@id\":\"https:\/\/www.adacyte.com\/#\/schema\/logo\/image\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Granulocyte and Monocyte Adsorption Apheresis Therapy Modulates Monocyte-Derived Dendritic Cell Function in Patients With Ulcerative Colitis - Adacyte","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.adacyte.com\/professional\/scientific-corner\/granulocyte-and-monocyte-adsorption-apheresis-therapy-modulates-monocyte-derived-dendritic-cell-function-in-patients-with-ulcerative-colitis\/","og_locale":"de_DE","og_type":"article","og_title":"Granulocyte and Monocyte Adsorption Apheresis Therapy Modulates Monocyte-Derived Dendritic Cell Function in Patients With Ulcerative Colitis - Adacyte","og_description":"Yuko Iwakami,Atsushi Sakuraba,Toshiro Sato,Yasuhiro Takada,Motoko Izumiya,Hitoshi Ichikawa,Toshifumi Hibi Ther Apher Dial 13,&nbsp;2 (2009);&nbsp;138-146 The aim of this study was to elucidate the molecular mechanisms responsible for the therapeutic effects of granulocyte and monocyte adsorption apheresis (GMA). 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