{"id":5829,"date":"1995-07-28T10:22:00","date_gmt":"1995-07-28T10:22:00","guid":{"rendered":"https:\/\/www.adacyte.com\/?post_type=scientific-corner&#038;p=5829"},"modified":"2021-07-28T10:26:14","modified_gmt":"2021-07-28T10:26:14","slug":"distinct-tyrosine-kinase-activation-and-triton-x-100-insolubility-upon-fc%ce%b3rii-or-fc%ce%b3riiib-ligation-in-human-polymorphonuclear-leukocytes","status":"publish","type":"scientific-corner","link":"https:\/\/www.adacyte.com\/de\/professional\/scientific-corner\/distinct-tyrosine-kinase-activation-and-triton-x-100-insolubility-upon-fc%ce%b3rii-or-fc%ce%b3riiib-ligation-in-human-polymorphonuclear-leukocytes\/","title":{"rendered":"Distinct Tyrosine Kinase Activation and Triton X-100 Insolubility upon Fc\u03b3RII or Fc\u03b3RIIIB Ligation in Human Polymorphonuclear Leukocytes."},"content":{"rendered":"<div class=\"post-content max-w-987 mx-auto px-20 lg:px-0\">\n<ul class=\"wp-block-list\"><li><a href=\"https:\/\/www.jbc.org\/article\/S0021-9258(18)92313-6\/fulltext#\">Ming-jie Zhou Douglas M. Lublin<\/a> <a href=\"https:\/\/www.jbc.org\/article\/S0021-9258(18)92313-6\/fulltext#\">Daniel C. Link<\/a> <a href=\"https:\/\/www.jbc.org\/article\/S0021-9258(18)92313-6\/fulltext#\">Eric J. Brown<\/a> <a href=\"https:\/\/www.jbc.org\/article\/S0021-9258(18)92313-6\/fulltext#\">Show<\/a>, DOI:<a href=\"https:\/\/doi.org\/10.1074\/jbc.270.22.13553\">https:\/\/doi.org\/10.1074\/jbc.270.22.13553<\/a><\/li><\/ul>\n<\/div>\n\n<div class=\"post-content max-w-987 mx-auto px-20 lg:px-0\">\n<p>Two tyrosine kinase-dependent pathways exist for activation of the respiratory burst by polymorphonuclear leukocyte (PMN) immunoglobulin G Fc receptors. Direct ligation of Fc\u03b3RII activates the respiratory burst, but ligation of the glycan phosphoinositol-linked Fc\u03b3RIIIB does not. Instead, this receptor and the integrin complement receptor CR3 synergize in activation of the respiratory burst (Zhou, M.-J., and Brown, E. J. (1994)\u00a0<em>J. Cell Biol.<\/em>\u00a0125, 1407-1416). Here we show that direct ligation of Fc\u03b3RII leads to activation and Triton X-100 insolubility of the Src family kinase Fgr, without effect on the related myeloid Src family member Hck. In contrast, adhesion of PMN via Fc\u03b3RIIIB leads to activation and Triton X-100 insolubility of Hck but not Fgr. The exclusive association of Fc\u03b3RIIIB with Hck activation and Triton insolubility is not solely a result of its glycan phosphoinositol anchor, since decay accelerating factor (CD55), another prominent glycan phosphoinositol-anchored PMN protein, is associated with Fgr insolubility to a greater extent than Hck. Ligation of decay accelerating factor, with or without coligation of CR3, does not activate the PMN respiratory burst. Coligation of Fc\u03b3RIIIB with Fc\u03b3RII overcomes the pertussis toxin inhibition of H<sub>2<\/sub>O<sub>2<\/sub>\u00a0production in response to direct ligation of Fc\u03b3RII. These data support the hypothesis that activation of Hck upon Fc\u03b3RIIIB ligation has a role in generation of the synergistic respiratory burst.<\/p>\n<\/div>\n\n<div class=\"post-content max-w-987 mx-auto px-20 lg:px-0\">\n<p><a href=\"https:\/\/www.jbc.org\/article\/S0021-9258(18)92313-6\/fulltext#%20\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.jbc.org\/article\/S0021-9258(18)92313-6\/fulltext#%20<\/a><\/p>\n<\/div>\n\n<div class=\"post-content max-w-987 mx-auto px-20 lg:px-0\">\n<p><a href=\"https:\/\/www.jbc.org\/article\/S0021-9258(18)92312-4\/fulltext\"><\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Ming-jie Zhou Douglas M. Lublin Daniel C. Link Eric J. Brown Show, DOI:https:\/\/doi.org\/10.1074\/jbc.270.22.13553 Two tyrosine kinase-dependent pathways exist for activation of the respiratory burst by polymorphonuclear leukocyte (PMN) immunoglobulin G Fc receptors. Direct ligation of Fc\u03b3RII activates the respiratory burst, but ligation of the glycan phosphoinositol-linked Fc\u03b3RIIIB does not. Instead, this receptor and the integrin [&hellip;]<\/p>\n","protected":false},"template":"","scientific_tags":[],"class_list":["post-5829","scientific-corner","type-scientific-corner","status-publish","hentry"],"acf":{"sc_authors":""},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Distinct Tyrosine Kinase Activation and Triton X-100 Insolubility upon Fc\u03b3RII or Fc\u03b3RIIIB Ligation in Human Polymorphonuclear Leukocytes. - Adacyte<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/distinct-tyrosine-kinase-activation-and-triton-x-100-insolubility-upon-fc\u03b3rii-or-fc\u03b3riiib-ligation-in-human-polymorphonuclear-leukocytes\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Distinct Tyrosine Kinase Activation and Triton X-100 Insolubility upon Fc\u03b3RII or Fc\u03b3RIIIB Ligation in Human Polymorphonuclear Leukocytes. - Adacyte\" \/>\n<meta property=\"og:description\" content=\"Ming-jie Zhou Douglas M. Lublin Daniel C. Link Eric J. Brown Show, DOI:https:\/\/doi.org\/10.1074\/jbc.270.22.13553 Two tyrosine kinase-dependent pathways exist for activation of the respiratory burst by polymorphonuclear leukocyte (PMN) immunoglobulin G Fc receptors. Direct ligation of Fc\u03b3RII activates the respiratory burst, but ligation of the glycan phosphoinositol-linked Fc\u03b3RIIIB does not. Instead, this receptor and the integrin [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/distinct-tyrosine-kinase-activation-and-triton-x-100-insolubility-upon-fc\u03b3rii-or-fc\u03b3riiib-ligation-in-human-polymorphonuclear-leukocytes\/\" \/>\n<meta property=\"og:site_name\" content=\"Adacyte\" \/>\n<meta property=\"article:modified_time\" content=\"2021-07-28T10:26:14+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Gesch\u00e4tzte Lesezeit\" \/>\n\t<meta name=\"twitter:data1\" content=\"2\u00a0Minuten\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/distinct-tyrosine-kinase-activation-and-triton-x-100-insolubility-upon-fc%ce%b3rii-or-fc%ce%b3riiib-ligation-in-human-polymorphonuclear-leukocytes\/\",\"url\":\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/distinct-tyrosine-kinase-activation-and-triton-x-100-insolubility-upon-fc%ce%b3rii-or-fc%ce%b3riiib-ligation-in-human-polymorphonuclear-leukocytes\/\",\"name\":\"Distinct Tyrosine Kinase Activation and Triton X-100 Insolubility upon Fc\u03b3RII or Fc\u03b3RIIIB Ligation in Human Polymorphonuclear Leukocytes. - Adacyte\",\"isPartOf\":{\"@id\":\"https:\/\/www.adacyte.com\/#website\"},\"datePublished\":\"1995-07-28T10:22:00+00:00\",\"dateModified\":\"2021-07-28T10:26:14+00:00\",\"inLanguage\":\"de\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.adacyte.com\/professional\/scientific-corner\/distinct-tyrosine-kinase-activation-and-triton-x-100-insolubility-upon-fc%ce%b3rii-or-fc%ce%b3riiib-ligation-in-human-polymorphonuclear-leukocytes\/\"]}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.adacyte.com\/#website\",\"url\":\"https:\/\/www.adacyte.com\/\",\"name\":\"Adacyte\",\"description\":\"Adacyte Therapeutics\",\"publisher\":{\"@id\":\"https:\/\/www.adacyte.com\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.adacyte.com\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"de\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.adacyte.com\/#organization\",\"name\":\"Adacyte\",\"url\":\"https:\/\/www.adacyte.com\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"de\",\"@id\":\"https:\/\/www.adacyte.com\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.adacyte.com\/wp-content\/uploads\/2021\/01\/logo-adacyte.svg\",\"contentUrl\":\"https:\/\/www.adacyte.com\/wp-content\/uploads\/2021\/01\/logo-adacyte.svg\",\"width\":165,\"height\":46,\"caption\":\"Adacyte\"},\"image\":{\"@id\":\"https:\/\/www.adacyte.com\/#\/schema\/logo\/image\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Distinct Tyrosine Kinase Activation and Triton X-100 Insolubility upon Fc\u03b3RII or Fc\u03b3RIIIB Ligation in Human Polymorphonuclear Leukocytes. - Adacyte","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.adacyte.com\/professional\/scientific-corner\/distinct-tyrosine-kinase-activation-and-triton-x-100-insolubility-upon-fc\u03b3rii-or-fc\u03b3riiib-ligation-in-human-polymorphonuclear-leukocytes\/","og_locale":"de_DE","og_type":"article","og_title":"Distinct Tyrosine Kinase Activation and Triton X-100 Insolubility upon Fc\u03b3RII or Fc\u03b3RIIIB Ligation in Human Polymorphonuclear Leukocytes. - Adacyte","og_description":"Ming-jie Zhou Douglas M. 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